Frederick C. Haer: An Introduction to Chromatography on Impregnated Glass Fiber.

Ann Arbor Science, Michigan, USA, 1969, 173 Seiten mit 76 Bildern. Gebunden Halbleinen.

In 1951 Kirschner introduced the technique of the chromatostrip which was improved by Stahl in 1956 and became thin-layer chromatography. Kirschner also developed a technique for impregnating cellulose paper with silicic acid. These two techniques were combined by Dieckert in 1954 into Silicic acid glass fiber paper chromatography. Dieckert also introduced the technique of spraying the chromatogram with sulfuric acid and then heating it to oxidize organic compounds to carbon. This is a technique par excellence for locating organic compounds on either glass fiber or thin-layer chromatograms. It was my good fortune to be present in Dr. Raymond Reiser's laboratory when Dieckert developed the technique and later, from 1957 to 1961, to have the opportunity of working with him. We published methods for bile acids and steroids and along with Swartwout developed the technique of quantitative char densitometry. This was later applied to thin-layer plates by Privett. Being a biologist interested in the metabolism of lipids, I am always looking for better, simpler, faster methods of separating lipids. Complex, tedious, slow methods bore me. A column of silicic acid, a thin layer of silica gel, a silicic acid impregnated cellulose paper, or a silica gel impregnated glass fiber paper can be expected to give approximately the same separation of a set of compounds. In this sense they are competitive. Glass fiber paper chromatography wins any argument about rapidity of separation. Commercial availability of impregnated papers has simplified the technique, but selection of one of the four techniques depends on the nature of the separation problem. For example, it may be necessary to isolate large amounts of material. In this case a silicic.